Properties of erythromycin-inducible transposon Tn917 in Streptococcus faecalis
نویسندگان
چکیده
منابع مشابه
Mapping of Streptococcus faecalis plasmids pAD1 and pAD2 and studies relating to transposition of Tn917.
Plasmids pAD1 (37.8 megadaltons) and pAD2 (17.1 megadaltons) of Streptococcus faecalis strain DS16 have been mapped with restriction enzymes. The location of a hemolysin-bacteriocin determinant on the conjugative pAD1 plasmid was derived from analyses of transposon insertions. Electron microscope and hybridization analyses located Tn917(Em) and the streptomycin (Sm) and kanamycin (Km) resistanc...
متن کاملTransposition of the gram-positive transposon Tn917 in Escherichia coli.
The streptococcal transposon Tn917 was demonstrated to transpose in Escherichia coli from the Bacillus subtilis-E. coli shuttle plasmid pHK1207 into an F' plasmid derivative. Subsequently, a second round of transposition from the F' plasmid into pACYC184 could be readily demonstrated. These results represent the initial demonstration of the transposition of a gram-positive transposon in a gram-...
متن کاملConstruction of an Enterococcus faecalis Tn917-mediated-gene-disruption library offers insight into Tn917 insertion patterns.
Sequencing the insertion sites of 8,865 Tn917 insertions in Enterococcus faecalis strain OG1RF identified a hot spot in the replication terminus region corresponding to 6% of the genome where 65% of the transposons had inserted. In E. faecalis, Tn917 preferentially inserted at a 29-bp consensus sequence centered on TATAA, a 5-bp sequence that is duplicated during insertion. The regional inserti...
متن کاملDetection of the Efflux-Mediated Erythromycin Resistance Transposon in Streptococcus pneumoniae
BACKGROUND The present analysis focuses on phenotypic and genotypic characterizations of efflux-mediated erythromycin resistance in Streptococcus pneumoniae due to an increase in macrolide resistance in S. pneumoniae worldwide. METHODS We investigated the prevalence of efflux-mediated erythromycin resistance and its relevant genetic elements from 186 specimens of S. pneumonia isolated from cl...
متن کاملTransposition of Tn917 in Bacillus megateriumt
Transposon Tn9l7 from Steptococcus faecalis (12) is presently the most useful transposon for Bacillus subtilis genetic manipulation. It is 5.3 kilobases in length, confers inducible erythromycin resistance, and has terminal inverted repeats very closely related to the Tn3 family of transposons of gram-negative organisms (11). Youngman et al. (16) constructed a Tn917-carrying plasmid, pTV1, that...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1980
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.141.3.1366-1374.1980